Beef protein is typically produced through a series of steps starting with the selection of high-quality beef, often sourced from lean cuts, which is then ground and homogenised to facilitate enzymatic action. Subsequently, the beef undergoes hydrolysis, where enzymes or acids break down the protein molecules into smaller peptides and amino acids, enhancing their bioavailability and digestibility.

Following hydrolysis, the mixture is filtered to remove insoluble components and impurities, before being dried using methods such as spray drying or freeze-drying to create a concentrated protein powder. This powder undergoes stringent quality control checks to ensure purity, potency, and safety before being packaged for distribution and consumption.

Variations in the manufacturing process may exist among different producers and brands, with some employing proprietary techniques to further enhance the nutritional profile and quality of their beef protein supplements.